Coverart for item
The Resource Apoptosis : a practical approach, edited by George P. Studzinski

Apoptosis : a practical approach, edited by George P. Studzinski

Label
Apoptosis : a practical approach
Title
Apoptosis
Title remainder
a practical approach
Statement of responsibility
edited by George P. Studzinski
Contributor
Subject
Genre
Language
eng
Summary
This text is designed to provide conceptual outlines and detailed procedures for basic and advanced studies of cell death by apoptosis. Chapters on the recognition of apoptosis as distinguished from necrosis and nonspecific cell DNA damage, are followed by a systematic examination of the established and the principal novel methodologies utilized by some leading laboratories conducting research on apoptosis. The organization is on the lines of signalling for apoptosis, the apoptotic cascade, and the execution of apoptosis. A wide variety of procedures are provided which will enable the reader to participate in cutting-edge research
Member of
Cataloging source
N$T
Dewey number
571.9/36
Illustrations
illustrations
Index
index present
LC call number
QH671
LC item number
.A6526 1999eb
Literary form
non fiction
NAL call number
QH671
NAL item number
.A6526 1999
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • 2000 F-400
  • QH 671
NLM item number
A64353 1999
http://library.link/vocab/relatedWorkOrContributorName
Studzinski, George P
Series statement
The practical approach series
Series volume
216
http://library.link/vocab/subjectName
  • Apoptosis
  • Apoptosis
  • SCIENCE
  • Apoptosis
  • Labortechnik
  • Apoptosis
  • Celdood
  • Biology
  • Health & Biological Sciences
  • Cytology
  • Apoptose
Label
Apoptosis : a practical approach, edited by George P. Studzinski
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Intro; Contents; List of Contributors; Abbreviations; 1. Overview of apoptosis; 1. General introduction and overview of contents; 2. Historical perspective; 3. Distinction of apoptosis from other forms of cell death; 4. Apoptotic cascades; 5. Time course of apoptotic cascades; 6. Selection of methods; Procedure for determination of increased mitochondrial to nuclear DNA ratio, for the detection and quantitation of apoptosis; 7. Pitfalls; References; 2. Morphological recognition of apoptotic cells; 1. Introduction; Key morphological features of apoptotic cells
  • 2. Light and fluorescent microscopy techniques for the assessment of apoptosisPreparation of cell or tissue samples; Nuclear counterstains; 3. Electron microscopic techniques; 4. Quantitation of apoptotic events; Methods; Problems in scoring apoptotic events; 5. In situ detection of DNA strand breaks; 6. Other techniques; 7. Conclusions; References; 3. Assessment of DNA damage in apoptosis; 1. Introduction; 2. Types of DNA fragmentation; 3. Measurement of high molecular weight DNA fragmentation by pulse-field gel electrophoresis; Pulse-field gel electrophoresis procedures
  • Strategies for electrophoretic separations4. Detection of internucleosomal DNA fragmentation by standard agarose gel electrophoresis; 5. Filter elution assay to measure apoptotic DNA fragmentation; Equipment; Filters and solutions; DNA labelling and preparation of experimental cell cultures; Protocols; Counting samples and computations; References; 4. Analysis of cell death by flow and laser-scanning cytometry; 1. Introduction; 2. Preparation of cells for analysis by LSC. Detection of apoptotic cells based on changes in nuclear chromatin
  • Fas-based cytotoxicityPerforin/granzyme-based cytotoxicity; 2. Standard methods for the evaluation of cell-mediated cytotoxicity; Chromium release assay; [[sup(125)]I]Iododeoxyuridine ([sup(125)]IUdR) release assay; The JAM test; BLT-esterase release assay; Estimation of target cell nuclear fragmentation using fluorescent dyes; Activation-induced cell death (AICD); 3. Separate studies of Fas- and perforin/granzyme-based cytotoxicity; Fas-based cytotoxicity in the absence of perforin/granzyme contribution; Perforin/granzyme-based cytotoxicity in the absence of FasL contribution
Control code
53957911
Dimensions
unknown
Extent
1 online resource (xxii, 244 pages)
File format
unknown
Form of item
online
Isbn
9781280374722
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)53957911
Label
Apoptosis : a practical approach, edited by George P. Studzinski
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Intro; Contents; List of Contributors; Abbreviations; 1. Overview of apoptosis; 1. General introduction and overview of contents; 2. Historical perspective; 3. Distinction of apoptosis from other forms of cell death; 4. Apoptotic cascades; 5. Time course of apoptotic cascades; 6. Selection of methods; Procedure for determination of increased mitochondrial to nuclear DNA ratio, for the detection and quantitation of apoptosis; 7. Pitfalls; References; 2. Morphological recognition of apoptotic cells; 1. Introduction; Key morphological features of apoptotic cells
  • 2. Light and fluorescent microscopy techniques for the assessment of apoptosisPreparation of cell or tissue samples; Nuclear counterstains; 3. Electron microscopic techniques; 4. Quantitation of apoptotic events; Methods; Problems in scoring apoptotic events; 5. In situ detection of DNA strand breaks; 6. Other techniques; 7. Conclusions; References; 3. Assessment of DNA damage in apoptosis; 1. Introduction; 2. Types of DNA fragmentation; 3. Measurement of high molecular weight DNA fragmentation by pulse-field gel electrophoresis; Pulse-field gel electrophoresis procedures
  • Strategies for electrophoretic separations4. Detection of internucleosomal DNA fragmentation by standard agarose gel electrophoresis; 5. Filter elution assay to measure apoptotic DNA fragmentation; Equipment; Filters and solutions; DNA labelling and preparation of experimental cell cultures; Protocols; Counting samples and computations; References; 4. Analysis of cell death by flow and laser-scanning cytometry; 1. Introduction; 2. Preparation of cells for analysis by LSC. Detection of apoptotic cells based on changes in nuclear chromatin
  • Fas-based cytotoxicityPerforin/granzyme-based cytotoxicity; 2. Standard methods for the evaluation of cell-mediated cytotoxicity; Chromium release assay; [[sup(125)]I]Iododeoxyuridine ([sup(125)]IUdR) release assay; The JAM test; BLT-esterase release assay; Estimation of target cell nuclear fragmentation using fluorescent dyes; Activation-induced cell death (AICD); 3. Separate studies of Fas- and perforin/granzyme-based cytotoxicity; Fas-based cytotoxicity in the absence of perforin/granzyme contribution; Perforin/granzyme-based cytotoxicity in the absence of FasL contribution
Control code
53957911
Dimensions
unknown
Extent
1 online resource (xxii, 244 pages)
File format
unknown
Form of item
online
Isbn
9781280374722
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
(OCoLC)53957911

Library Locations

    • Thomas Jefferson LibraryBorrow it
      1 University Blvd, St. Louis, MO, 63121, US
      38.710138 -90.311107
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