The Resource Effects of aflatoxin B1 (AFB1) on hepatic gene expression in pigs and turkeys, by Rafael Azevedo Murarolli

Effects of aflatoxin B1 (AFB1) on hepatic gene expression in pigs and turkeys, by Rafael Azevedo Murarolli

Label
Effects of aflatoxin B1 (AFB1) on hepatic gene expression in pigs and turkeys
Title
Effects of aflatoxin B1 (AFB1) on hepatic gene expression in pigs and turkeys
Statement of responsibility
by Rafael Azevedo Murarolli
Creator
Contributor
Author
Thesis advisor
Subject
Genre
Language
eng
Summary
The objective of the present study was to evaluate the efficacy of curcumin (CMN), an antioxidant supplied by turmeric (Curcuma longa) powder to ameliorate the adverse effects of aflatoxin B1 (AFB1) on performance of pigs and, to identify changes in gene expression in liver of pigs fed aflatoxin (AF). Twenty crossbred weanling pigs were weighed, ear-tagged, and assigned to each of four dietary treatments, which included: 1) basal diet (BD) containing no AFB1 or CMN; 2) BD + 1.0 mg AFB1/kg of diet; 3) BD + 100 mg curcumin (CMN)/kg of diet, and; 4) BD + 100 mg CMN/kg of diet + 1.0 mg AFB1/kg of diet. Aflatoxin reduced (P < 0.05) body weight gain (BWG), feed intake (FI) and feed efficiency of pigs. The addition of CMN to the diet contaminated with AFB1 improved feed efficiency (P < 0.05) but not BWG and FI. At the end of three week treatment period, livers were collected and microarray analysis was conducted to identify pathways that control growth, development, coagulation, immune function, metabolism, detoxification, and antioxidant status in liver of pigs. Genes with an adjusted permutation Fs test (false discovery rates) values less that 5% and fold change greater than 2.0 were considered differentially expressed across samples. Changes in expression were determined using microarray technique and results were validated using quantitative real time PCR (RTqPCR). Six genes were chosen for validation of expression using RT-qPCR, including TNF receptor superfamily, member 6 (FAS), glutathione S-transferase theta 1 (GSTT1), cyclin G1 (CCGN1), proteasome activator subunit 1 (PSME1), proteasome activator subunit 3 (PSME3), and cytochrome P450-2A19 (CYP2A19). There were no differences in the expression of the genes among the treatments except for GSTT1 and CYP2A19 that shifted the expression (down to up, and up to down regulation, respectively) with the addition of CMN in the diet contaminated with AFB1. Results demonstrate that pigs fed 1.0 mg AFB1/kg feed for 21 days had reduced growth performance associated with altered hepatic gene expression, and the supplementation of 100 mg CMN/kg to diets containing AFB1 had a protective effect of curcumin on changes in gene expression in liver of pigs
Cataloging source
MUU
http://library.link/vocab/creatorName
Murarolli, Rafael Azevedo
Degree
Ph.D.
Dissertation note
Dissertation
Dissertation year
2013.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorDate
1952-
http://library.link/vocab/relatedWorkOrContributorName
Ledoux, David R.
Label
Effects of aflatoxin B1 (AFB1) on hepatic gene expression in pigs and turkeys, by Rafael Azevedo Murarolli
Instantiates
Publication
Note
  • Abstract from short.pdf
  • "December 2013."
  • "A Dissertation presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment Of the Requirements for the Degree Doctor of Philosophy."
  • Dissertation supervisor: Dr. D. R. Ledoux
  • Includes vita
Bibliography note
Includes bibliographical references (pages 138-152)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
899214460
Extent
1 online resource (ix, 153 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)899214460
Label
Effects of aflatoxin B1 (AFB1) on hepatic gene expression in pigs and turkeys, by Rafael Azevedo Murarolli
Publication
Note
  • Abstract from short.pdf
  • "December 2013."
  • "A Dissertation presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment Of the Requirements for the Degree Doctor of Philosophy."
  • Dissertation supervisor: Dr. D. R. Ledoux
  • Includes vita
Bibliography note
Includes bibliographical references (pages 138-152)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
899214460
Extent
1 online resource (ix, 153 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)899214460

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