Coverart for item
The Resource Molecular biomethods handbook, edited by Ralph Rapley and John M. Walker

Molecular biomethods handbook, edited by Ralph Rapley and John M. Walker

Label
Molecular biomethods handbook
Title
Molecular biomethods handbook
Statement of responsibility
edited by Ralph Rapley and John M. Walker
Contributor
Subject
Language
eng
Summary
  • "In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few
  • Numerous key protein methods, as well as support and related techniques, are also included."--Jacket
Cataloging source
DLC
Dewey number
572.8
Illustrations
illustrations
Index
index present
LC call number
QH506
LC item number
.M6642 1998
Literary form
non fiction
Nature of contents
bibliography
NLM call number
QZ 50
NLM item number
M71832 1998
http://library.link/vocab/relatedWorkOrContributorDate
1948-
http://library.link/vocab/relatedWorkOrContributorName
  • Rapley, Ralph
  • Walker, John M.
http://library.link/vocab/subjectName
  • Molecular biology
  • Cytological Techniques
  • Genetic Techniques
  • Molecular Biology
Label
Molecular biomethods handbook, edited by Ralph Rapley and John M. Walker
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Extraction of total RNA from tissues and cultured cells / Sandeep Raha, Mingfu Ling and Frank Merante -- Isolation of total cellular DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, and Mingfu Ling -- Gel electrophoresis of DNA / Duncan R. Smith -- S1 nuclease protection assay (RPA) / Ralf Einspanier and Annette Plath -- Gene Probes / Marilena Aquino de Muro -- Nonradioactive labeling of DNA / Thomas P. McCreery and Terrance R. Barrette -- Southern blot analysis / Paolo A. Sabelli -- Northern blot analysis / Paolo A. Sabelli -- DNA sequencing / Chris Spencer -- Autoradiography and fluorography / Bronwen Harvey -- Mobility shift assays / Nigel J. Savery and Stephen J.W. Busby -- cDNA libraries / Ian G. Cowell -- Genomic DNA libraries / Bimal D.M. Theophilus -- [lambda] as a cloning vector / Rupert Mutzel -- Plasmid-derived cloning vectors / Craig Winstanley and Ralph Rapley -- M13 and phagemid-based cloning vectors / Ralph Rapley -- Retroviral vectors : from laboratory tools to moleculare medicines / Richard G. Vile, Anna Tuszynski and Simon Castledon -- Baculovirus vectors / Azeem Ansari and Vincent C. Emery
  • Gene transfer and expression in tissue culture cells of higher eukaryotes / M. Alexandra Aitken, Selina Raguz and Michael Antoniou -- Plant transformation / Andy Prescott, Rob Briddon and Wendy Harwood -- Restriction fragment-length ploymorphisms / Elaine K. Green -- Genome mapping / Jacqueline Boultwood -- Yeast artificial chromosomes / Angela Flannery and Rakesh Anand -- Polymerase chain reaction / Ralph Rapley -- In vitro transcription / Martin J. Tymms -- Site-directed mutagenesis / John R. Adair and T. Paul Wallace -- Transgenic techniques / Roberta M. James and Paul Dickinson -- DNA profiling : theory and practice / Karen M. Sullivan -- Radiolabeling of peptides and proteins / Arvind C. Patel and Stewart R. Mattewson -- Protein electrophoresis / David J. Begley -- Protein blotting : principles and applications / Peter R. Shewry and Roger J. Fido -- Ion-exchange chromatography / Paul Cutler -- Hydrophobic interaction chromatography / Paul A. O'Farrell -- Affinity chromatography / George W. Jack -- Reversed-phase HPLC / Bill Neville -- Glycoprotein analysis / Terry D. Butters -- Protein sequencing / Bryan J. Smith and John R. Chapman -- Solid-phase peptide synthesis / Gregg B. Fields -- Protein engineering / Sudhir Paul -- Monoclonal antibodies / Chritopher Dean and Helmout Modjtahedi -- Phage-display libraries / Julie E. Thompson and Andrew J. Williams -- Enzyme-linked immunosorbent assay (ELISA) / John R. Crowther -- Epitope mapping : identification of antibody binding sites on protein antigens / Glenn E. Morris -- Immunocytochemistry / Lorette C. Javois -- Flow cytometry / Robert E. Cunningham -- Mass spectrometry / John R. Chapman -- The technique of In situ hybridization : principles and applications / Desire du Sart and K.H. Andy Choo
Control code
39856415
Dimensions
26 cm
Extent
xiii, 725 pages
Isbn
9780896035010
Isbn Type
(alk. paper)
Lccn
98226406
Media category
unmediated
Media MARC source
rdamedia
Media type code
n
Other physical details
illustrations
Label
Molecular biomethods handbook, edited by Ralph Rapley and John M. Walker
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
volume
Carrier category code
nc
Carrier MARC source
rdacarrier
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Extraction of total RNA from tissues and cultured cells / Sandeep Raha, Mingfu Ling and Frank Merante -- Isolation of total cellular DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, and Mingfu Ling -- Gel electrophoresis of DNA / Duncan R. Smith -- S1 nuclease protection assay (RPA) / Ralf Einspanier and Annette Plath -- Gene Probes / Marilena Aquino de Muro -- Nonradioactive labeling of DNA / Thomas P. McCreery and Terrance R. Barrette -- Southern blot analysis / Paolo A. Sabelli -- Northern blot analysis / Paolo A. Sabelli -- DNA sequencing / Chris Spencer -- Autoradiography and fluorography / Bronwen Harvey -- Mobility shift assays / Nigel J. Savery and Stephen J.W. Busby -- cDNA libraries / Ian G. Cowell -- Genomic DNA libraries / Bimal D.M. Theophilus -- [lambda] as a cloning vector / Rupert Mutzel -- Plasmid-derived cloning vectors / Craig Winstanley and Ralph Rapley -- M13 and phagemid-based cloning vectors / Ralph Rapley -- Retroviral vectors : from laboratory tools to moleculare medicines / Richard G. Vile, Anna Tuszynski and Simon Castledon -- Baculovirus vectors / Azeem Ansari and Vincent C. Emery
  • Gene transfer and expression in tissue culture cells of higher eukaryotes / M. Alexandra Aitken, Selina Raguz and Michael Antoniou -- Plant transformation / Andy Prescott, Rob Briddon and Wendy Harwood -- Restriction fragment-length ploymorphisms / Elaine K. Green -- Genome mapping / Jacqueline Boultwood -- Yeast artificial chromosomes / Angela Flannery and Rakesh Anand -- Polymerase chain reaction / Ralph Rapley -- In vitro transcription / Martin J. Tymms -- Site-directed mutagenesis / John R. Adair and T. Paul Wallace -- Transgenic techniques / Roberta M. James and Paul Dickinson -- DNA profiling : theory and practice / Karen M. Sullivan -- Radiolabeling of peptides and proteins / Arvind C. Patel and Stewart R. Mattewson -- Protein electrophoresis / David J. Begley -- Protein blotting : principles and applications / Peter R. Shewry and Roger J. Fido -- Ion-exchange chromatography / Paul Cutler -- Hydrophobic interaction chromatography / Paul A. O'Farrell -- Affinity chromatography / George W. Jack -- Reversed-phase HPLC / Bill Neville -- Glycoprotein analysis / Terry D. Butters -- Protein sequencing / Bryan J. Smith and John R. Chapman -- Solid-phase peptide synthesis / Gregg B. Fields -- Protein engineering / Sudhir Paul -- Monoclonal antibodies / Chritopher Dean and Helmout Modjtahedi -- Phage-display libraries / Julie E. Thompson and Andrew J. Williams -- Enzyme-linked immunosorbent assay (ELISA) / John R. Crowther -- Epitope mapping : identification of antibody binding sites on protein antigens / Glenn E. Morris -- Immunocytochemistry / Lorette C. Javois -- Flow cytometry / Robert E. Cunningham -- Mass spectrometry / John R. Chapman -- The technique of In situ hybridization : principles and applications / Desire du Sart and K.H. Andy Choo
Control code
39856415
Dimensions
26 cm
Extent
xiii, 725 pages
Isbn
9780896035010
Isbn Type
(alk. paper)
Lccn
98226406
Media category
unmediated
Media MARC source
rdamedia
Media type code
n
Other physical details
illustrations

Library Locations

    • Thomas Jefferson LibraryBorrow it
      1 University Blvd, St. Louis, MO, 63121, US
      38.710138 -90.311107
Processing Feedback ...