The Resource Multi-frequency electrical impedance method for detection of viable micro-organisms, their quantification and their characterization, by Sachidevi Puttaswamy

Multi-frequency electrical impedance method for detection of viable micro-organisms, their quantification and their characterization, by Sachidevi Puttaswamy

Label
Multi-frequency electrical impedance method for detection of viable micro-organisms, their quantification and their characterization
Title
Multi-frequency electrical impedance method for detection of viable micro-organisms, their quantification and their characterization
Statement of responsibility
by Sachidevi Puttaswamy
Creator
Contributor
Author
Thesis advisor
Subject
Genre
Language
eng
Summary
Despite advances in DNA based technologies and molecular methods, there are still several drawbacks to these methods and hence, there is a need for development of a new technology for microbial detection and quantification. We present here a novel method for detecting the presence of viable microorganisms in various suspensions ranging from bacterial growth media, to food substrates such as milk and apple juice, water and complex suspensions like blood along with their quantification and characterization. The underlying principle for our method of detection of microorganisms is based on the fact that in the presence of an AC electric field, the intact/viable bacterial cell membrane becomes polarized. This polarization leads to the buildup of charges across the intact membrane and hence, these viable microbial cells behave like electrical capacitors. An increase in the number of microorganisms due to proliferation thus results in a corresponding increase in the "bulk capacitance" of the suspension containing bacteria, due to an increase in the total amount of charge stored in the interior of a suspension. However, this increase cannot be directly measured since the capacitance of the solid-liquid interface ("double layer" which is usually 1̃000X larger than the bulk capacitance) in effect "screens" the former. In the work presented here, we introduce a method that is able to discern such changes with high sensitivity and robustness. We also demonstrate the ability of our method to a) Monitor food quality/safety by detecting bacterial proliferation in "real world" liquid food samples like milk and apple juice b) Quantify the bacteria in a sample by estimating the MPN of the viable bacteria c) Detect presence of viable bacteria in blood for diagnosis of Sepsis d) Determine the Minimum Inhibitory Concentrations (MIC) and assay for bactericidal/bacteriostatic activity of various bacteria e) Extend the application to detect presence of other microorganisms such as yeast and mold
Cataloging source
MUU
http://library.link/vocab/creatorName
Puttaswamy, Sachidevi
Degree
Ph.D.
Dissertation note
Dissertation
Dissertation year
2013.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Sengupta, Shramik
http://library.link/vocab/subjectName
  • Microorganisms
  • Cell membranes
  • Molecular microbiology
Label
Multi-frequency electrical impedance method for detection of viable micro-organisms, their quantification and their characterization, by Sachidevi Puttaswamy
Instantiates
Publication
Copyright
Note
  • "December 2013."
  • "A Dissertation Presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in Biological Engineering."
  • Dissertation supervisor: Dr. Shramik Sengupta
  • Vita
Bibliography note
Includes bibliographical references (pages 153-160)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
895190503
Extent
1 online resource (x, 161 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)895190503
Label
Multi-frequency electrical impedance method for detection of viable micro-organisms, their quantification and their characterization, by Sachidevi Puttaswamy
Publication
Copyright
Note
  • "December 2013."
  • "A Dissertation Presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in Biological Engineering."
  • Dissertation supervisor: Dr. Shramik Sengupta
  • Vita
Bibliography note
Includes bibliographical references (pages 153-160)
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent.
Control code
895190503
Extent
1 online resource (x, 161 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
  • c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)895190503

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