The Resource Rapid isolation of target bacteria from complex matrices, by Jinwang Tan

Rapid isolation of target bacteria from complex matrices, by Jinwang Tan

Label
Rapid isolation of target bacteria from complex matrices
Title
Rapid isolation of target bacteria from complex matrices
Statement of responsibility
by Jinwang Tan
Creator
Contributor
Author
Thesis advisor
Subject
Genre
Language
eng
Summary
The long term goal of the research effort in our group was to develop a new rapid diagnostic process for sepsis. The specific project described in this work aims to obtain these "pure" isolates of the infectious bacteria within a total time of less than 1.5 hours. The proposed isolation process consists of 2 steps: (a) a novel kinetically limited density differential centrifugation step that serves as a "coarse" method, and (b): a dielectrophoresis (DEP) based sorting technique that serves as a "polishing" step. Kinetically Limited Density Differential Centrifugation enables us to convert a mixture in which the bacteria are an extremely small fraction of the particles to a suspension where bacteria constitute the majority of the particles. It is expected that the sample yielded by this separation technique can be "polished" using flow through dielectrophoresis (DEP). We also measured the DEP properties of target bacteria and RBC to determine the experiment condition for further purification. Besides that, we designed and fabricated the MEMS device for Flow-Through Dielectrophoresis (DEP) to obtain pure isolates of bacteria
Cataloging source
MUU
http://library.link/vocab/creatorName
Tan, Jinwang
Degree
M.S.
Dissertation note
Thesis
Dissertation year
2010.
Government publication
government publication of a state province territory dependency etc
Granting institution
University of Missouri--Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Sengupta, Shramik
Label
Rapid isolation of target bacteria from complex matrices, by Jinwang Tan
Instantiates
Publication
Note
  • "July 2010."
  • "A Thesis presented to the Faculty of the Graduate School at the University of Missouri In Partial Fulfillment of the Requirements for the Degree Master of Science."
  • Thesis supervisor: Dr. Shramik Sengupta
Bibliography note
Includes bibliographical references (pages 78-80)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
txt
Content type MARC source
rdacontent.
Control code
900642985
Extent
1 online resource (viii, 80 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)900642985
Label
Rapid isolation of target bacteria from complex matrices, by Jinwang Tan
Publication
Note
  • "July 2010."
  • "A Thesis presented to the Faculty of the Graduate School at the University of Missouri In Partial Fulfillment of the Requirements for the Degree Master of Science."
  • Thesis supervisor: Dr. Shramik Sengupta
Bibliography note
Includes bibliographical references (pages 78-80)
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier.
Content category
text
Content type code
txt
Content type MARC source
rdacontent.
Control code
900642985
Extent
1 online resource (viii, 80 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia.
Media type code
c
Other physical details
illustrations (some color)
Specific material designation
remote
System control number
(OCoLC)900642985

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