The Resource Separation and identification of peptides by integrated multidimensional liquid chromatography-mass spectrometry (IMDLC-MS), by Harika Adusumilli, (electronic resource)

Separation and identification of peptides by integrated multidimensional liquid chromatography-mass spectrometry (IMDLC-MS), by Harika Adusumilli, (electronic resource)

Label
Separation and identification of peptides by integrated multidimensional liquid chromatography-mass spectrometry (IMDLC-MS)
Title
Separation and identification of peptides by integrated multidimensional liquid chromatography-mass spectrometry (IMDLC-MS)
Statement of responsibility
by Harika Adusumilli
Creator
Contributor
Thesis advisor
Subject
Genre
Language
eng
Summary
Integrated multidimensional liquid chromatography (IMDLC) is an innovative method used for separation of tryptic peptides. In this method a two-component column, containing strong cation exchange (SCX) and reverse-phase (RP) were used for two-dimensional liquid chromatography. To separate peptides on an SCX column two salts were used: ammonium formate and ammonium acetate. The salt was injected onto the column in the form of pulse in 13 different steps ranging from 0 to 100% through a quaternary pumping system. This novel method was tested on model peptides, small proteins and Arabidopsis thaliana proteins extracted from leaves. The proteins were digested with trypsin and the obtained tryptic peptides were used for the separation process. A total of 118 proteins were identified in Arabidopsis thaliana leaves. The two salts were compared and a larger number of peptides were identified when formate was used. This method is designed on a conventional HPLC with a quaternary pump yielding two dimensional separations
Cataloging source
MUU
http://library.link/vocab/creatorName
Adusumilli, Harika
Degree
M.S.
Dissertation year
2007.
Granting institution
University of Missouri-Columbia
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • theses
http://library.link/vocab/relatedWorkOrContributorName
Greenlief, Michael
http://library.link/vocab/subjectName
  • Peptides
  • Ammonium salts
  • Arabidopsis thaliana
  • Liquid chromatography
  • Mass spectrometry
  • High performance liquid chromatography
  • Cations
Target audience
specialized
Label
Separation and identification of peptides by integrated multidimensional liquid chromatography-mass spectrometry (IMDLC-MS), by Harika Adusumilli, (electronic resource)
Instantiates
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on April 15, 2008)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
223967547
Dimensions
unknown
Form of item
electronic
Governing access note
Access is limited to the campuses of the University of Missouri
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Specific material designation
remote
System control number
(OCoLC)223967547
System details
Mode of access: World Wide Web
Label
Separation and identification of peptides by integrated multidimensional liquid chromatography-mass spectrometry (IMDLC-MS), by Harika Adusumilli, (electronic resource)
Publication
Note
  • The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file
  • Title from title screen of research.pdf file (viewed on April 15, 2008)
  • Vita
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
mixed
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Control code
223967547
Dimensions
unknown
Form of item
electronic
Governing access note
Access is limited to the campuses of the University of Missouri
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Specific material designation
remote
System control number
(OCoLC)223967547
System details
Mode of access: World Wide Web

Library Locations

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      38.710138 -90.311107
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