Cover -- Half-title -- Title -- Copyright -- Dedication -- Contents -- Preface -- Acknowledgments -- 1 Introduction -- Further reading -- 2 Producing gametes -- Oocyte growth -- Follicle development -- Storing information -- The regional organization of the oocyte: polarization -- Oogenesis in the human -- Meiotic arrest and resumption of meiosis -- Resumption of meiosis -- Spermatogenesis in mammals -- Further reading -- 3 Sperm-oocyte interaction -- The acrosome and the vitelline coat -- Sperm-oocyte fusion -- Activation of the spermatozoon -- Motility -- Chemotaxis -- Capacitation
Acrosome reaction -- Sperm-oocyte interaction in mammals -- Capacitation in mammals -- Acrosome reaction -- Sperm-oocyte fusion -- Oocyte activation -- Releasing meiotic arrest -- 1. The G-protein hypothesis -- 2. The soluble sperm factor hypothesis -- Intracellular calcium release -- Calcium and the cell cycle proteins -- The cortical reaction -- The cortical reaction in mammals -- The role of cortical reorganization -- Fusion, centrosomes and pronuclei -- Centrosomes -- Formation of pronuclei -- Pronucleus formation in mammals -- Syngamy -- 'The sun in the egg' -- Further reading
4 First stages of development -- Activation of the zygote genome -- Imprinting -- Compaction -- Causes of embryo arrest -- Paternal factors -- Metabolic requirements of the early mammalian embryo in vitro Yves Ménézo -- Cleavage patterns -- Cytoplasmic segregation and the formation of cell lines -- Further reading -- 5 Endocrine control of reproduction -- Further reading -- 6 Assisted reproductive technology in farm animals -- Artificial insemination -- Bovine IVF -- (with thanks to R. Brittain) -- Maturation (IVM) -- Fertilization (IVF) -- Embryo culture (IVC) -- IVF during pregnancy
Results -- The large calf syndrome -- Pregnancy results -- Sex distribution of in vitro produced calves -- Gender selection -- Microsurgery in mammalian embryos -- Further reading -- 7 The clinical in vitro fertilization laboratory -- Introduction -- Setting up a laboratory: equipment and facilities -- Ambient air quality -- Laboratory space layout -- Incubators -- Water quality -- Supplies -- Daily cleaning routine -- Washing procedures -- Pipettes -- Non-disposable items (handle with non-powered gloves, rinsed in purified water) -- Tissue culture media -- Serum -- Growth factors
Follicular flushing -- Quality control procedures -- Suggested useful routine QC procedures include the following: -- 1. Sperm survival test -- 2. Culture of surplus oocytes -- 3. Multipronucleate embryo culture -- 4. Culture of 'spare' embryos -- Tissue culture systems -- Oil preparation -- Serum supplements -- Preparation of maternal serum -- Basic equipment required for the IVF laboratory -- Embryology -- Details of IVF media can be obtained from the following manufacturing companies: -- Further reading -- 8 Semen analysis and preparation for assisted reproductive techniques

Control code: 51202596

Dimensions: unknown

Edition: 2nd ed.

Extent: 1 online resource (xii, 310 pages)

Form of item: online

Isbn: 9780511006029

Media category: computer

Media MARC source: rdamedia

Media type code

Other physical details: illustrations

http://library.link/vocab/ext/overdrive/overdriveId: ebl144670

Record ID: .b131066602

Specific material designation: remote

System control number: (OCoLC)51202596

Context

Context of In vitro fertilization, Kay Elder, Brian Dale

Context

Item

Instantiates